Bacillus cereus Agar Base with Egg Yolk Emulsion and Polymyxin

[所属分类:培养基配方] [发布时间:2019-11-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus cereus Agar Base

with Egg Yolk Emulsion and Polymyxin
Composition per liter:
Agar............................................................................................15.0g
Sodium pyruvate.........................................................................10.0g
Mannitol......................................................................................10.0g
Na 2 HPO 4 .......................................................................................2.5g
NaCl..............................................................................................2.0g
Peptone .........................................................................................1.0g
KH 2 PO 4 .......................................................................................0.25g
Bromthymol Blue.......................................................................0.12g
MgSO 4 ·7H 2 O................................................................................0.1g
Egg yolk emulsion.................................................................100.0mL
Selective supplement solution.................................................10.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without egg yolk emulsion, is available as a
premixed powder from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Polymyxin B....................................................................... 100,000 U
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Egg Yolk Emulsion:
Composition per liter:
Egg yolks.................................................................................30.0mL
NaCl, 0.9% solution.................................................................70.0mL
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack 11 eggs
and separate yolks from whites. Mix egg yolks. Measure 30.0mL of
egg yolk emulsion and add to 70.0mL of 0.9% sterile NaCl solution.
Mix thoroughly. Warm to 45°–50°C.
Preparation of Medium:  Add components, except egg yolk emul-
sion, and selective supplement solution, to distilled/deionized water
and bring volume to 890.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.  Aseptically add 100.0mL egg yolk emulsion and
10.0mL sterile selective supplement solution. Mix well.  Pour
into sterile Petri dishes or sterile tubes.
Use: For the isolation, detection, and enumeration of Bacillus
cereus.