Bacillus cereus HiVeg Agar Base with Egg Yolk Emulsion

[所属分类:培养基配方] [发布时间:2019-11-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus cereus HiVeg Agar Base
with Egg Yolk Emulsion
Composition per liter:
Agar .......................................................................15.0g
Sodium pyruvate.............................................................10.0g
Mannitol....................................................................10.0g
Na2HPO4 .....................................................................2.5g
NaCl.........................................................................2.0g
Plant peptone................................................................1.0g
KH2PO4 .....................................................................0.25g
Bromthymol Blue .............................................................0.12g
MgSO 4 ·7H 2 O.................................................................0.1g
Egg yolk emulsion.................................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without egg yolk emulsion, is available as a
premixed powder from HiMedia.
Egg Yolk Emulsion:
Composition per liter:
Egg yolks .....................................................................30.0mL
NaCl, 0.9% solution.............................................................70.0mL
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack 11 eggs
and separate yolks from whites. Mix egg yolks. Measure 30.0mL of
egg yolk emulsion and add to 70.0mL of 0.9% sterile NaCl solution.
Mix thoroughly. Warm to 45°–50°C.
Preparation of Medium:  Add components, except egg yolk emu-
lusion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL
egg yolk emulsion. Mix well. Pour into sterile Petri dishes or sterile
tubes.
Use: For the isolation, detection, and enumeration of Bacillus
cereus.