Bacillus cereus Medium (BCM)

[所属分类:培养基配方] [发布时间:2019-11-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus cereus Medium
(BCM)
Composition per 110.0mL:
Agar ...............................................................................2.0g
D- Mannitol.........................................................................1.0g
(NH4)2PO4 ..........................................................................0.1g
KCl................................................................................0.02g
MgSO4 ·7H2O........................................................................0.02g
Yeast extract................................................................................0.02g
Bromcresol Purple .....................................................................4.0mg
Egg yolk emulsion, 20%..........................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Egg Yolk Emulsion, 20%:
Composition per 100.0mL:
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................80.0mL
Preparation of Egg Yolk Emulsion, 20%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Measure 20.0mL of egg yolk emulsion and add to 80.0mL of 0.9%
NaCl solution. Mix thoroughly. Filter sterilize. Warm to 45°–50°C.
Preparation of Medium: Add components—except egg yolk
emulsion, 20%—to distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 10.0mL of sterile egg yolk emulsion, 20%. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Bacillus cereus.