Bacillus filiformis Medium (DSMZ Medium 992)

[所属分类:培养基配方] [发布时间:2019-11-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus filiformis Medium
(DSMZ Medium 992)
Composition per liter:
Yeast extract................................................................10.0g
Sodium citrate................................................................3.0g
KCl...........................................................................2.0g
MgSO4 ·7H2O..................................................................1.0g
Sodium chloride solution...................................................100.0mL
Sodium carbonate solution...................................................10.0mL
Iron sulfate solution........................................................1.0mL
Manganese chloride solution..................................................1.0mL
pH 9.0 ± 0.2 at 25°C
Iron Sulfate Solution:
Composition per liter:
FeSO4 ·7H2O..................................................................50.0g
Preparation of Iron Sulfate Solution:  Add FeSO4 ·7H2O to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sodium Chloride Solution:
Composition per 100.0mL:
NaCl........................................................................100.0g
Preparation of Sodium Chloride Solution:  Add NaCl to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Manganese Chloride Solution:
Composition per liter:
MnCl2 ·4H2O ................................................................0.36g
Preparation of Manganese Chloride Solution:  Add MnCl2 ·4H2O
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Sodium Carbonate Solution:
Composition per 10.0mL:
Na2CO3 ...................................................................3.0g
Preparation of Sodium Carbonate Solution:  Add Na2CO3 to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except sodium chloride
and sodium carbonate solutions, to distilled/deionized water and bring
volume to 890.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally add 100.0mL sterile sodium chloride solution and 10.0mL sterile
sodium carbonate solution. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation of a Bacillus filiformis.