Bacillus halodenitrificans Agar (LMG Medium 142)

[所属分类:培养基配方] [发布时间:2019-11-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus halodenitrificans Agar
(LMG Medium 142)
Composition per liter:
NaCl..........................................................................................100.0g
Agar............................................................................................15.0g
Sodium acetate·3H2O..................................................................10.0g
Na2HPO4 .......................................................................................3.8g
KH2PO4 .........................................................................................1.3g
(NH4)2SO4 ....................................................................................1.0g
Mg(NO3)2 ·6H2O...........................................................................1.0g
Yeast extract..................................................................................1.0g
Magnesium nitrate solution...................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Magnesium Nitrate Solution:
Composition per 100.0mL:
Mg(NO3)2·6H2O...........................................................................1.0g
Preparation of Magnesium Nitrate Solution: Add Mg(NO3)2·6H2O
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except magnesium ni-
trate solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Adjust pH to 7.2 with KOH. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
100.0mL sterile magnesium nitrate solution. Mix thoroughly. Asepti-
cally pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Bacillus halodenitrificans.