Bacillus mascerans Medium (TSBY Salt Medium) (LMG 199)

[所属分类:培养基配方] [发布时间:2019-11-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus mascerans Medium
(TSBY Salt Medium)
(LMG 199)
Composition per liter:
NaCl.......................................................................18.0g
Pancreatic digest of casein................................................17.0g
MgCl2 ·H2O.................................................................4.0g
MgSO4 ·7H20...............................................................3.45g
Yeast extract...............................................................3.0g
Papaic digest of soybean meal...............................................3.0g
K2HPO4 .....................................................................2.5g
Glucose ....................................................................2.5g
KCl........................................................................0.34g
NH4Cl .....................................................................0.25g
CaCl2 ·2H2O...............................................................0.14g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Bacillus mascerans, Carnobacterium alter-
funditum, and Carnobacterium funditum.