Bacillus schlegelii Agar

[所属分类:培养基配方] [发布时间:2019-11-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bacillus schlegelii Agar
Composition per liter:
Noble agar....................................................30.0g
Na2HPO4 ·2H2O.................................................4.5g
KH2PO4 ........................................................1.5g
NH4Cl .........................................................1.0g
MgSO4 ·7H2O...................................................0.2g
MnSO4 ·H2O...................................................0.01g
CaCl2 ·2H2O..................................................0.01g
Ferric ammonium citrate.......................................5.0mg
Agar solution...............................................200.0mL
Pyruvate solution ..........................................100.0mL
Vrace elements solution SL-6 ................................3.0mL
pH 7.1 ± 0.2 at 25°C
Agar Solution:
Composition per 200.0mL:
Noble agar...................................................30.0g
Preparation of Agar Solution: Add agar to distilled/deionized
water and bring volume to 200.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Pyruvate Solution:
Composition per 100.0mL:
Sodium pyruvate................................................1.5g
Preparation of Pyruvate Solution: Add sodium pyruvate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize. Warm to 45°–50°C.
Trace Elements Solution SL-6 :
Composition per liter:
H3BO3 ...........................................................0.3g
CoCl2 ·6H2O.....................................................0.2g
ZnSO4 ·7H2O.....................................................0.1g
MnCl2 ·4H2  ...................................................0.03g
Na2MoO4 ·H2O...................................................0.03g
NiCl2 ·6H2O....................................................0.02g
CuCl2 ·2H2O....................................................0.01g
Preparation of Trace Elements Solution SL-6 : Add components
to distilled/deionized water and bring volume to 1.0L. Adjust pH to 3.4.
Preparation of Medium: Add components, except sodium pyru-
vate solution and agar solution, to distilled/deionized water and bring
volume to 700.0mL. Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 7.1. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°C. Add sodium pyruvate solution and agar solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Bacillus schlegelii.