Bacillus schlegelii Broth

[所属分类:培养基配方] [发布时间:2019-11-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bacillus schlegelii Broth
Composition per liter:
Na2HPO4 ·2H2O....................................................4.5g
KH2PO4 ...........................................................1.5g
NH4Cl ............................................................1.0g
MgSO4 ·7H2O......................................................0.2g
MnSO4 ·H2O......................................................0.01g
CaCl2 ·2H2O ....................................................0.01g
Ferric ammonium citrate..........................................5.0mg
Pyruvate solution..............................................100.0mL
SL-6 trace elements ............................................3.0mL
pH 7.1 ± 0.2 at 25°C
Pyruvate Solution:
Composition per 100.0mL:
Sodium pyruvate...................................................1.5g
Preparation of Pyruvate Solution: Add sodium pyruvate to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Trace Elements Solution SL-6 :
Composition per liter:
H3BO3 ............................................................0.3g
CoCl2 ·6H2O......................................................0.2g
ZnSO4 ·7H2O......................................................0.1g
MnCl2 ·4H2O ....................................................0.03g
Na2MoO4 ·H2O....................................................0.03g
NiCl2 ·6H2O.....................................................0.02g
CuCl2 ·2H2O.....................................................0.01g
Preparation of Trace Elements Solution SL-6 : Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 3.4.
Preparation of Medium: Add components, except sodium pyru-
vate solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH
to 7.1. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add sodium pyruvate solution. Aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation and maintenance of Bacillus schlegelii.