Bacillus schlegelii Chemolithotrophic Growth Medium (DSMZ Medium 261)

[所属分类:培养基配方] [发布时间:2019-12-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus schlegelii Chemolithotrophic Growth Medium
(DSMZ Medium 261)

培养基配方
Composition per liter:
Na2HPO4 ·2H2O.....................................................4.5g
KH2PO4 ............................................................1.5g
NH4Cl .............................................................1.0g
MgSO4 ·7H2O.......................................................0.2g
MnSO4 ·2H2O......................................................0.01g
CaCl2 ·2H2O......................................................0.01g
Ferric ammonium citrate...........................................5.0mg
Trace elements solution SL-6 .....................................3.0mL
pH 7.1 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O........................................................0.5g
H3BO3 ..............................................................0.3g
CoCl2 ·6H2O .......................................................0.2g
ZnSO4 ·7H2O....................................................... 0.1g
Na2MoO4 ·2H2O ................................................... 0.03g
NiCl2 ·6H2O.......................................................0.02g
CuCl2 ·2H2O ......................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the chemolithotrophic cultivation of Bacillus schlegelii. Incu-
bation is at 65°C under an atmosphere of 5% O2 , 10% CO2 , 45% H2.