Bacillus schlegelii Heterotrophic Growth Medium (DSMZ Medium 260)

[所属分类:培养基配方] [发布时间:2019-12-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus schlegelii Heterotrophic Growth Medium
(DSMZ Medium 260)

培养基配方
Composition per liter:
Na2HPO4 ·2H2O.................................................4.5g
Na-pyruvate ...................................................1.5g
KH2PO4 ........................................................1.5g
NH4Cl .........................................................1.0g
MgSO4 ·7H2O...................................................0.2g
MnSO4 ·2H2O..................................................0.01g
CaCl2 ·2H2O..................................................0.01g
Ferric ammonium citrate.......................................5.0mg
Trace elements solution SL-6 .................................3.0mL
pH 7.1 ± 0.2 at 25°C
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O......................................................0.5g
H3BO3 ............................................................0.3g
CoCl2 ·6H2O .....................................................0.2g
ZnSO4 ·7H2O......................................................0.1g
Na2MoO4·2H2O....................................................0.03g
NiCl2 ·6H2O.....................................................0.02g
CuCl2 ·2H2O.....................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the heterotrophic cultivation of Bacillus schlegelii. Incubation
is at 65°C.