Bacillus schlegelii Medium (LMG Medium 85)

[所属分类:培养基配方] [发布时间:2019-12-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus schlegelii Medium
(LMG Medium 85）

培养基配方
Composition per liter:
Na2HPO4 ·12H2O.................................................9.0g
KH2PO4 .........................................................1.5g
Sodium pyruvate.................................................1.5g
NH4Cl ..........................................................1.0g
MgSO4 ·7H2O....................................................0.2g
MnSO4 ·H2O...................................................10.0mg
CaCl2 ·2H2O .................................................10.0mg
Ferric ammonium citrate........................................5.0mg
Trace elements solution........................................3.0mL
pH 7.1 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
H3BO3 .......................................................0.3g
CoCl2 ·6H2O.................................................0.2g
ZnSO4 ·7H2O.................................................0.1g
Na2MoO4 ·2H2O.............................................30.0mg
MnCl2 ·4H2O ..............................................30.0mg
NiCl2 ·6H2O...............................................20.0mg
CuCl2 ·2H2O...............................................10.0mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute in 30–50
mL amounts in Erlenmeyer flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. Incubate without agitation.
Use: For the cultivation of Bacillus schlegelii.