Benzene Sulfonate Medium

[所属分类:培养基配方] [发布时间:2019-12-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Benzene Sulfonate Medium

培养基配方
Composition per liter:
Agar...........................................................15.0g
Sodium benzene sulfonate........................................1.0g
(NH4)2SO4 ......................................................1.0g
K2HPO4 .........................................................0.7g
KH2PO4 .........................................................0.3g
MgSO4 ·7H2O....................................................0.2g
CaCl2 ........................................................10.0mg
FeSO4 ·7H2O...................................................5.0mg
ZnSO4 ·7H2O.................................................70.0μg
CuSO4 .......................................................50.0μg
H3BO3 .......................................................10.0μg
MoO3 ·2H2O..................................................10.0μg
MnSO4 ·5H2O..................................................2.0μg
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat gently to boil-
ing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Comamonas testosteroni.