Benzoate Medium

[所属分类:培养基配方] [发布时间:2019-12-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Benzoate Medium

培养基配方
Composition per liter:
Noble agar...............................................20.0g
NaCl......................................................5.0g
(NH4)2HPO4 ...............................................3.0g
Sodium benzoate...........................................3.0g
KH2PO4 ...................................................1.2g
Yeast extract.............................................0.5g
MgSO4 ·7H2O..............................................0.2g
Benzoate solution.......................................25.0mL
Benzoate Solution:
Composition per 25.0mL:
Sodium benzoate...........................................3.0g
Preparation of Benzoate Solution: Add sodium benzoate to dis-
tilled/deionized water and bring volume to 25.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components except benzoate solu-
tion to distilled/deionized water and bring volume to 975.0mL. Mix
thoroughly. Heat gently to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 25.0mL sterile benzo-
ate solution. Mix thoroughly and pour into sterile Petri dishes or leave
in tubes.
Use:  For the cultivation of Pseudomonas putida and other microor-
ganisms which can utilize benzoate as a carbon source.