Benzoate Medium II

[所属分类:培养基配方] [发布时间:2019-12-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Benzoate Medium II

培养基配方
Composition per 1.5L:
Noble agar..................................................30.0g
(NH4)2HPO4 ..................................................3.0g
NaCl........................................................1.67g
KH2PO4 ......................................................1.2g
Yeast extract................................................0.5g
MgSO4 ·7H2O.................................................0.2g
FeSO4 ·7H2O.................................................0.1g
Benzoate solution..........................................25.0mL
Benzoate Solution:
Composition per 25.0mL:
Sodium benzoate..............................................1.0g
Preparation of Benzoate Solution: Add sodium benzoate to dis-
tilled/deionized water and bring volume to 25.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium:  Add components, except agar and sodium
benzoate, to distilled/deionized water and bring volume to 600.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. In a separate flask, add agar to distilled/deionized water and
bring volume to 375.0mL. Mix thoroughly. Gently heat and bring to boil-
ing. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically combine the two autoclave-sterilized solutions. Mix thor-
oughly. Aseptically add the sterile benzoate solution. Mix thoroughly.
Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation of Pseudomonas putida and other microor-
ganisms that can utilize benzoate as a carbon source.