BG 11 Medium (Medium BG 11 for Cyanobacteria)

[所属分类:培养基配方] [发布时间:2019-12-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BG 11 Medium
(Medium BG 11 for Cyanobacteria)

培养基配方
Composition per liter:
Agar.......................................................10.0g
NaNO3 ......................................................1.5g
MgSO4 ·7H2O..............................................0.075g
K2HPO4 ....................................................0.04g
CaCl2 ·2H2O .............................................0.036g
Na2CO3 ....................................................0.02g
Citric acid................................................6.0mg
Ferric ammonium citrate....................................6.0mg
EDTA disodium salt.........................................1.0mg
Trace metal mix A5 ........................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Metal Mix A5:
Composition per liter:
H3BO3 .....................................................2.86g
MnCl2 ·4H2O ..............................................1.81g
Na2MoO4 ·2H2O.............................................0.39g
ZnSO4 ·7H2O..............................................0.222g
CuSO4 ·5H2O..............................................0.079g
Co(NO3)2 ·6H2O ..........................................0.049g
Preparation of Trace Metal Mix A5:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Anabaena species, Calo-
thrix species, Chaemisiphon species, Chorogloeopsis species, Chroo-
coccidiopsis species, Crinalium epipsammum, Cylindrospermum spe-
cies, Dermocarpa species, Fischerella species, Gloebacter violaceus,
Gloeocapsa species, Gloeothece species, Hapalosiphon fontinalis,
Nostoc species, Oscillatoria species, Phormidium species, Pleuro-
capsa species, Pseudanabaena species, Scytonema species, Spirulina
species, Synechococcus species, Synechocystis species, and Tolypo-
thrix tenuis.