BG 11 Uracil Agar

[所属分类:培养基配方] [发布时间:2019-12-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BG 11 Uracil Agar

培养基配方
Composition per liter:
Agar ..........................................................10.0g
Uracil .........................................................2.8g
NaNO3 ..........................................................1.5g
MgSO4 ·7H2O..................................................0.075g
K2HPO4 ........................................................0.04g
CaCl2 ·2H2O..................................................0.036g
Na2CO3 ........................................................0.02g
Citric acid....................................................6.0mg
Ferric ammonium citrate........................................6.0mg
EDTA disodium salt.............................................1.0mg
Trace metal mix A5.............................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Metal Mix A5:
Composition per liter:
H3BO3 .........................................................2.86g
MnCl2 ·4H2O...................................................1.81g
Na2MoO4 ·2H2O ................................................0.39g
ZnSO4 ·7H2O..................................................0.222g
CuSO4 ·5H2O..................................................0.079g
Co(NO3)2 ·6H2O ..............................................0.049g
Preparation of Trace Metal Mix A5: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat gently to boil-
ing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C. Pour into sterile Petri dishes.
Use:  For the cultivation and maintenance of Anabaena variabilis.