BHI with Glycerol and Reducing Agents (DSMZ Medium 215c)

[所属分类:培养基配方] [发布时间:2019-12-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BHI with Glycerol and Reducing Agents
(DSMZ Medium 215c)

培养基配方
Composition per liter:
Pancreatic digest of gelatin..................................14.5g
Brain heart, solids from infusion .............................6.0g
Peptic digest of animal tissue.................................6.0g
NaCl...........................................................5.0g
Glucose .......................................................3.0g
Na2HPO4 .......................................................2.5g
Glycerol solution............................................10.0mL
L- Cysteine·HCl–Na2S solution..............................10.0mL
pH 7.4 ± 0.2 at 25°C
Glycerol Solution:
Composition  per 100.0mL:
Glycerol ....................................................87.0g
Preparation of Glycerol Solution: Add glycerol to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly.
L -Cysteine·HCl–Na2S Solution:
Composition per 100.0mL:
L- Cysteine·HCl.............................................2.5g
Na2S·9H2O...................................................2.5g
Preparation of  L -Cysteine·HCl–Na2S Solution: Add  L -
cysteine·HCl to distilled/deionized water and bring volume to 80.0mL.
Mix thoroughly. Adjust pH to 11 with NaOH. Add Na2S·9H2O. Mix
thoroughly. Bring volume to 100.0mL with distilled/deionized water.
Gently heat and bring to boiling under 100% N2 . Cool to 25°C under
100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except  L -cysteine·HCl–
Na2S solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling under 100% N2 . Cool to
25°C under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Aseptically and anaerobically under 100% N2  add 10.0mL of  L -
cysteine·HCl–Na2S solution. Mix thoroughly. Aseptically under 100% N2
distribute to tubes. Alternately distribute 10.0mL amounts of the medium
without  L -cysteine·HCl–Na2S solution to tubes prior to autoclaving. Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically and anaerobically
add 1.0mL of  L -cysteine·HCl–Na2S solution to each tube.
Use: For the cultivation of Clostridium sp.