BHI Medium (DSMZ Medium 215)

[所属分类:培养基配方] [发布时间:2019-12-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BHI Medium
(DSMZ Medium 215)

培养基配方
Composition per liter:
Pancreatic digest of gelatin.................................14.5g
Brain heart, solids from infusion ............................6.0g
Peptic digest of animal tissue................................6.0g
NaCl..........................................................5.0g
Na2HPO4 ......................................................2.5g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Enterococcus hirae, Lodobacter fluviatilis,
Yersinia spp., Tatumella ptyseos, Mycobacterium vanbaalenii, Oli-
gella urethralis=Moraxella urethralis, Moraxella (Branhamella)
catarrhalis, Campylobacter sputorum, Helcococcus kunzii, Bacillus
sporothermodurans, Haemophilus actinomycetemcomitans, Escheri-
chia coli, Pelczaria aurantia, Bacillus spp., Comamonas nitrativorans,
Salmonella bongori (Salmonella choleraesuis subsp. bongori), Sphin-
gomonas sanguinis (Sphingomonas sanguis), Arsenophonus nasoniae,
Streptococcus orisratti, Listeria spp., Jonesia denitrificans=Listeria
denitrificans, Propionibacterium propionicus=Arachnia propionica,
Corynebacterium spp., and Nocardiopsis tropica.