Blue-Green Broth

[所属分类:培养基配方] [发布时间:2019-12-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blue-Green Broth

培养基配方
Composition per liter:
NaNO3 .....................................................1.5g
MgSO4 ·7H2O.............................................0.075g
K2HPO4 ...................................................0.04g
CaCl2 ·2H2O.............................................0.036g
Na2CO3 ...................................................0.02g
Citric acid...............................................6.0mg
Ferric ammonium citrate...................................6.0mg
EDTA disodium salt........................................1.0mg
Vitamin B 12 solution....................................50.0mL
Trace metal mix A5 .......................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Metal Mix A5:
Composition per liter:
H3BO3 ...................................................2.86g
MnCl2 ·4H2O ............................................1.81g
Na2MoO4 ·2H2O...........................................0.39g
ZnSO4 ·7H2O............................................0.222g
CuSO4 ·5H2O............................................0.079g
Co(NO3)2 ·6H2O.........................................0.049g
Preparation of Trace Metal Mix A5: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin B 12 Solution:
Composition per 50.0mL:
Vitamin B 12 ..........................................0.01g
Preparation of Vitamin B 12 Solution: Add vitamin B 12 solution
to distilled/deionized water and bring volume to 50.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except vitamin B 12 , to
glass distilled water and bring volume to 950.0mL. Mix thoroughly.
Heat gently and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool the basal medium to 45°–50°C. Add vitamin B 12 so-
lution. Mix thoroughly. Distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Synechococcus species.