Blue-Green Nitrogen-Fixing Agar

[所属分类:培养基配方] [发布时间:2019-12-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blue-Green Nitrogen-Fixing Agar

培养基配方
Composition per liter:

Noble agar...............................................10.0g
MgSO4 ·7H2O............................................0.075g
K2HPO4 ..................................................0.04g
CaCl2 ·2H2O ...........................................0.036g
Na2CO3 ..................................................0.02g
Citric acid..............................................6.0mg
Ferric ammonium citrate..................................6.0mg
EDTA disodium salt.......................................1.0mg
Trace metal mix A5 ......................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Metal Mix A5:
Composition per liter:
H3BO3 ...................................................2.86g
MnCl2 ·4H2O ............................................1.81g
Na2MoO4 ·2H2O...........................................0.39g
ZnSO4 ·7H2O............................................0.222g
CuSO4 ·5H2O............................................0.079g
Co(NO3)2 ·6H2O.........................................0.049g
Preparation of Trace Metal Mix A5: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to glass-distilled water
and bring volume to 1.0L. Mix thoroughly. Heat gently and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Check pH af-
ter autoclaving and readjust if necessary. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Calothrix, Fischerella,
and Nostoc species.