Blue-Green Nitrogen-Fixing Broth

[所属分类:培养基配方] [发布时间:2019-12-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blue-Green Nitrogen-Fixing Broth

培养基配方
Composition per liter:
MgSO4 ·7H2O...........................................0.075g
K2HPO4 .................................................0.04g
CaCl2 ·2H2O...........................................0.036g
Na2CO3 .................................................0.02g
Citric acid.............................................6.0mg
Ferric ammonium citrate.................................6.0mg
EDTA disodium salt......................................1.0mg
Trace metal mix A5......................................1.0mL
pH 7.1 ± 0.2 at 25°C
Trace Metal Mix A5:
Composition per liter:
H3BO3 .................................................2.86g
MnCl2 ·4H2O...........................................1.81g
Na2MoO4 ·2H2O ........................................0.39g
ZnSO4 ·7H2O..........................................0.222g
CuSO4 ·5H2O..........................................0.079g
Co(NO3)2 ·6H2O ......................................0.049g
Preparation of Trace Metal Mix A5: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to glass-distilled water
and bring volume to 1.0L. Mix thoroughly. Heat gently and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Check pH af-
ter autoclaving and readjust if necessary. Aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation and maintenance of Calothrix, Fischerella,
and Nostoc species.