BMPA-α Medium (Semiselective Medium for Legionella pneumophila)

[所属分类:培养基配方] [发布时间:2019-12-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BMPA-α Medium
(Semiselective Medium for Legionella pneumophila)

培养基配方
Composition per liter:
Agar.....................................................15.0g
Yeast extract............................................10.0g
ACES buffer (2-[(2-amino-2-oxoethyl)-
amino]-ethane sulfonic acid).............................10.0g
Charcoal, activated.......................................2.0g
α-Ketoglutarate..........................................1.0g
Fe4(P2O7)3 ·9H2O........................................0.25g
Antibiotic inhibitor ...................................10.0mL
L- Cysteine·HCl·H2O solution..........................10.0mL
pH 6.9 ± 0.2 at 25°C
Antibiotic Inhibitor:
Composition per 10.0mL:
Anisomycin...............................................0.08g
Cefamandole..............................................4.0mg
Polymyxin B............................................80,000U
Preparation of Antibiotic Inhibitor: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
L - Cysteine·HCl·H2O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H2O..................................0.4g
Preparation of  L -Cysteine·HCl·H2O Solution: Add  L-
cysteine·HCl·H2O to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except antibiotic inhib-
itor and  L- cysteine·HCl·H2O solution , to distilled/deionized water and
bring volume to 980.0mL. Mix thoroughly. Adjust medium to pH 6.9
with 1N KOH. Heat gently and bring to boiling for 1 min. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Add 10.0mL
of the sterile  L -cysteine·HCl·H2O solution and 10.0mL of the sterile
antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes with
constant agitation to keep charcoal in suspension.
Use: For the selective isolation and cultivation of Legionella pneumophila
and other Legionella species.