BMS Agar

[所属分类:培养基配方] [发布时间:2019-12-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BMS Agar

培养基配方
Composition per liter:
Agar ......................................................20.0g
L -Malic acid...............................................2.5g
Sucrose.....................................................2.5g
KOH.........................................................2.0g
Potato extract solution .................................950.0mL
Bromthymol Blue ...........................................1.0mL
Vitamin solution...........................................1.0mL
pH 7.0 ± 0.2 at 25°C
Potato Extract Solution:
Composition per liter:
Potatoes, washed, peeled, and sliced .....................200.0g
Preparation of Potato Extract Solution:  Wash, peel, and slice
several large potatoes. Place the poltato slices in a gauze bag. Place the
bag with the potatoes in 1.0L of distilled/deionized water. Boil for 30
min. Filter through cotton.
Bromthymol Blue Solution:
Composition per 100.0mL:
Bromthymol Blue ............................................0.5g
Ethanol, 95%.............................................100.0mL
Preparation of Bromthymol Blue Solution:  Add Bromthymol
Blue to 100.0mL of 95% ethanol. Mix thorougly.
Vitamin Solution:
Composition per 100.0mL:
Biotin.....................................................10.0g
Pyridoxine................................................20.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium:  Dissolve 2.5g of  L -malic acid in 50.0mL of
distilled/deionized water. Add 1.0mL of Bromthymol Blue solution. Ad-
just pH to 7.0 by adding KOH so that the solution is green. Add 950.0mL
of potato extract solution. Mix thoroughly. Add 20.0g of agar and 2.5g of
sucrose. Mix thoroughly. Gently heat and bring to boiling. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add
1.0mL of sterile vitamin solution. Mix thoroughly. Pour into sterile Petri
dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Azospirillum lipoferum.