Bordet-Gengou Medium (LMG Medium 23)

[所属分类:培养基配方] [发布时间:2020-1-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bordet-Gengou Medium
(LMG Medium 23)

培养基配方
Composition per liter:
Agar ........................................................20.0g
Proteose peptone.............................................10.0g
NaCl..........................................................5.5g
Pancreatic digest of casein...................................5.0g
Peptic digest of animal tissue................................5.0g
Potato, solids from 125g infusion.............................4.5g
Rabbit blood, sterile defibrinated.........................150.0mL
Glycerol ...................................................10.0mL
pH 6.7± 0.2 at 25°C
Preparation of Medium: Add 10.0mL of glycerol to 850.0mL of
distilled/deionized water. Add the other components, except rabbit
blood, to the glycerol solution. Mix thoroughly. Heat with occasional
agitation of the medium. Boil for 1 min. Autoclave for 15 min at 15 psi
pressure–121°C. Cool medium to 45–50°C. Aseptically add 150.0mL
of rabbit blood (prewarmed to 35°C). Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the detection and isolation of Pseudomonas pertucinogena.