Borrelia Medium

[所属分类:培养基配方] [发布时间:2020-1-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Borrelia Medium

培养基配方
Composition per 370.0mL:
Solution 4.................................................240.0mL
Solution 1..................................................80.0mL
Solution 2..................................................34.0mL
Rabbit serum, sterile.......................................10.0mL
Solution 3..................................................4.0mL
Solution 5..................................................0.7mL
Solution 1:
Composition per liter:
Na2HPO 4 ·7H 2 O.........................................26.52g
Glucose...................................................12.75g
Proteose peptone No.2......................................5.95g
Pancreatic digest of casein................................2.55g
NaCl........................................................1.2g
Sodium pyruvate............................................1.06g
NaH2PO4 ·H2O..............................................1.03g
KCl........................................................0.85g
MgCl2 ·6H2O ..............................................0.68g
N-acetylglucosamine........................................0.53g
Sodium citrate·2H2O.......................................0.47g
Preparation of Solution 1: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Store at −20° C.
Solution 2:
Composition per 100.0mL:
Bovine albumin fraction V ..................................10.0g
Preparation of Solution 2: Add bovine albumin to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Adjust pH
to 7.8 with NaOH. Store at −20°C.
Solution 3:
Composition per 100.0mL:
NaHCO3 ................................................4.5g
Preparation of Solution 3:  Add NaHCO 3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Prepare solution
freshly.
Solution 4:
Composition per 100.0mL:
Gelatin..................................................7.0g
Preparation of Solution 4: Add gelatin to distilled/deionized water
and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min
at 10 psi pressure–115°C. Store at 4°C.
Solution 5:
Composition per 100.0mL:
Phenol Red..............................................0.5g
Preparation of Solution 5: Add Phenol Red to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Store at 4°C.
Preparation of Medium: Combine 80.0mL of solution 1, 34.0mL of
solution 2, 4.0mL of solution 3, 0.7mL of solution 5, and 1.3mL of dis-
tilled/deionized water. Mix thoroughly. Filter sterilize under pressure.
Aseptically distribute into sterile borosilicate screw-capped tubes in
6.0mL volumes. Melt solution 4 by immersing tube in warm water. Add
2.0mL of solution 4 to each screw-capped tube. Add 0.5mL of sterile
rabbit serum to each screw-capped tube.
Use: For the cultivation of Borrelia hermsii, Borrelia turicatae, and
Borrelia parkeri.