Bovine Serum Albumin Tween™ 80 Broth (BSA Tween™ 80 Broth)

[所属分类:培养基配方] [发布时间:2020-1-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bovine Serum Albumin Tween™ 80 Broth
(BSA Tween™ 80 Broth)

培养基配方
Composition per liter:
Basal medium..................................................900.0mL
Albumin supplement............................................100.0mL
pH 7.4 ± 0.2 at 25°C
Basal Medium:
Composition per liter:
Na2HPO4 .....................................................1.0g
NaCl.........................................................1.0g
KH2PO4 ......................................................0.3g
Glycerol (10% solution) ....................................1.0mL
NH4Cl (25% solution) .......................................1.0mL
Sodium pyruvate (10% solution)..............................1.0mL
Thiamine (0.5% solution ....................................1.0mL
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to
7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Albumin Supplement:
Composition per 100.0mL:
Bovine albumin.............................................10.0g
Tween ™ 80 (10% solution).................................12.5mL
FeSO4 (0.5% solution).....................................10.0mL
MgCl2 –CaCl 2 solution....................................1.0mL
Cyanocobalamin (0.02% solution) ...........................1.0mL
ZnSO4 (0.4% solution)......................................1.0mL
Preparation of Albumin Supplement: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Adjust pH to 7.4. Filter sterilize.
MgCl2 –CaCl2 Solution:
Composition per 100.0mL:
CaCl2 ·2H2O................................................1.5g
MgCl2 ·6H2O................................................1.5g
Preparation of MgCl 2 –CaCl 2 Solution: Add components to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: To 900.0mL of cooled, sterile basal me-
dium, aseptically add 100.0mL of sterile albumin supplement. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Leptospira species.