Bovine Albumin Tween™ 80 Medium, Ellinghausen and McCullough, Modified (Albumin Fatty Acid Broth, Leptospira Medium)

[所属分类:培养基配方] [发布时间:2020-1-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bovine Albumin Tween™ 80 Medium,
Ellinghausen and McCullough, Modified
(Albumin Fatty Acid Broth,
Leptospira Medium)

培养基配方
Composition per liter:
Basal medium .........................................900.0mL
Albumin fatty acid supplement.........................100.0mL
Basal Medium:
Composition per liter:
Na 2 HPO 4 , anhydrous ................................1.0g
NaCl...................................................1.0g
KH 2 PO 4 , anhydrous..................................0.3g
NH 4 Cl (25% solution)................................1.0mL
Glycerol (10% solution)...............................1.0mL
Sodium pyruvate (10% solution)........................1.0mL
Thiamine·HCl (0.5% solution).........................1.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Basal Medium: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to
7.4. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C.
Albumin Fatty Acid Supplement:
Composition per 200.0mL:
Bovine albumin fraction V ...........................20.0g
Polysorbate (Tween™) 80 (10% solution)..............25.0mL
FeSO4 ·7H2O (0.5% solution)........................20.0mL
CaCl2 ·2H2O (1.5% solution)........................2.0mL
MgCl2 ·2H2O (1.5% solution)........................2.0mL
Vitamin B 12 (0.2% solution)........................2.0mL
ZnSO4 ·7H2O (0.4% solution) .......................2.0mL
CuSO4 ·5H2O (0.3% solution)........................0.2mL
Preparation of Albumin Fatty Acid Supplement:  Add bovine
albumin to 100.0mL of distilled/deionized water. Mix thoroughly. Add
remaining components while stirring. Adjust pH to 7.4. Bring volume
to 200.0mL with distilled/deionized water. Filter sterilize. Store this
supplement at −20°C.
Preparation of Medium:  Aseptically combine 100.0mL of sterile
albumin fatty acid supplement and 900.0mL of sterile basal medium.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Leptospira species.