Brain Heart CC Agar, HiVeg (Brain Heart Cycloheximide Chloramphenicol Agar, HiVeg)

[所属分类:培养基配方] [发布时间:2020-1-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart CC Agar, HiVeg
(Brain Heart Cycloheximide
Chloramphenicol Agar, HiVeg)

培养基配方
Composition per liter:
Agar ..........................................................15.0g
Plant infusion.................................................10.0g
Plant peptone No.3.............................................10.0g
Plant special infusion..........................................7.5g
NaCl............................................................5.0g
Na2HPO4 ........................................................2.5g
Glucose ........................................................2.0g
Cycloheximide...................................................0.5g
Chloramphenicol................................................0.05g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks while shaking to distribute precipitate. Autoclave for 15 min
at 15 psi pressure–118°C.
Use: For the selective isolation of fastidious pathogenic fungi such as
Histoplasma capsulatum and Blastomyces dermatiditis from speci-
mens heavily contaminated with bacteria and other fungi. This medium
may also be used as a base supplemented with sheep blood and gentam-
icin for enrichment and additional selectivity.