Brain Heart Infusion (BHI)

[所属分类:培养基配方] [发布时间:2020-1-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion
(BHI)

培养基配方
Composition per liter:
Pancreatic digest of gelatin................................14.5g
Brain heart, solids from infusion............................6.0g
Peptic digest of animal tissue ..............................6.0g
NaCl.........................................................5.0g
Glucose......................................................3.0g
Na2HPO4 .....................................................2.5g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath and BD Diagnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of fastidious and nonfastidious microorganisms,
including aerobic and anaerobic bacteria, from a variety of clinical and
nonclinical specimens. It is particularly useful for culturing streptococci,
pneumococci, and meningococci. It is also used for the preparation of
inocula for use in antimicrobial susceptibility tests and as a base for blood
culture.