Brain Heart Infusion Agar (BHI Agar)

[所属分类:培养基配方] [发布时间:2020-1-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar
(BHI Agar)

培养基配方
Composition per liter:
Beef heart infusion.........................................250.0g
Calf brain infusion.........................................200.0g
Agar.........................................................13.5g
Proteose peptone.............................................10.0g
NaCl..........................................................5.0g
Na2HPO4 ·12H2O...............................................2.5g
Glucose.......................................................2.0g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of a variety of fastidious and nonfastidious aer-
obic and anaerobic microorganisms.