Brain Heart Infusion Agar

[所属分类:培养基配方] [发布时间:2020-1-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar

培养基配方

Composition per liter:
Pancreatic digest of casein................................16.0g
Agar.......................................................13.5g
Brain heart, solids from infusion...........................8.0g
Peptic digest of animal tissue .............................5.0g
NaCl........................................................5.0g
Glucose.....................................................2.0g
Na2HPO4 ....................................................2.5g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath and BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks while shaking to distribute precipitate. Autoclave for 15 min
at 15 psi pressure–121°C.
Use:  For the cultivation of a wide variety of fastidious microorganisms,
including bacteria, yeasts, and molds. With the addition of 10% sheep
blood, it is used for the isolation and cultivation of many fungal species,
including systemic fungi, from clinical and nonclinical specimens. The
addition of gentamicin and chloramphenicol with 10% sheep blood pro-
duces a selective medium used for the isolation of pathogenic fungi from
specimens heavily contaminated with bacteria and saprophytic fungi. It is
recommended for the isolation of Histoplasma capsulatum and other
pathogenic fungi, including Coccidioides immitis.