Brain Heart Infusion Agar with 1% Agar, HiVeg with Penicillin

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with 1% Agar, HiVeg
with Penicillin

培养基配方
Composition per liter:
Agar ......................................................15.0g
Plant infusion.............................................10.0g
Plant peptone No.3.........................................10.0g
Plant special infusion......................................7.5g
NaCl........................................................5.0g
Na2HPO4 ....................................................2.5g
Glucose ....................................................2.0g
Penicillin solution........................................2.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Penicillin Solution:
Composition per 2.0mL:
Penicillin.................................................0.1g
Preparation of Penicillin Solution: Add penicillin to ethanol and
bring volume to 2.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45–
50°C. Aseptically add 2.0mL penicillin solution. Mix thoroughly. Pour
into sterile Petri dishes or leave in tubes.
Use: For the cultivation of a variety of fastidious pathogenic bacteria,
yeasts, and molds.