Brain Heart Infusion Agar with Chloramphenicol

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with Chloramphenicol

培养基配方
Composition per liter:
Pancreatic digest of casein..............................16.0g
Agar ....................................................13.5g
Brain heart, solids from infusion ........................8.0g
Peptic digest of animal tissue............................5.0g
NaCl......................................................5.0g
Glucose ..................................................2.0g
Na2HPO4 ..................................................2.5g
Sheep blood, defibrinated...............................50.0mL
Chloramphenicol solution................................10.0mL
pH 7.4 ± 0.2 at 25°C
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol....................................0.05g
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except chlorampheni-
col solution and sheep blood, to distilled/deionized water and bring
volume to 940.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile chloramphenicol solution and sheep blood. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of a wide variety of fungal species,
especially systemic fungi, from clinical and nonclinical specimens. For
the selective isolation of pathogenic fungi from specimens heavily con-
taminated with bacteria and saprophytic fungi. For the maintenance of
fungal species on slant cultures.