Brain Heart Infusion Agar with Cysteine (LMG Medium 282)

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with Cysteine
(LMG Medium 282)

培养基配方
Composition per 1010.1mL:
Brain heart infusion agar .................................1.0L
L -Cysteine solution.....................................10.0mL
pH 7.4 ± 0.2 at 25°C
Brain Heart Infusion Agar:
Composition per liter:
Beef heart infusion.....................................250.0g
Calf brain infusion.....................................200.0g
Agar.....................................................13.5g
Proteose peptone.........................................10.0g
NaCl......................................................5.0g
Na2HPO4 ·12H2O...........................................2.5g
Glucose...................................................2.0g
Preparation of Brain Heart Infusion Agar: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
L -Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H2O.........................................0.5g
Preparation of  L -Cysteine Solution:  Add  L -cysteine·HCl·H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium: Aseptically add 10.0mL sterile  L -
cysteine solution to 1.0L sterile brain heart infusion agar. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Clostridium lactatifermentans.