Brain Heart Infusion Agar with Kanamycin

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with Kanamycin

培养基配方
Composition per liter:
Pancreatic digest of casein................................16.0g
Agar.......................................................13.5g
Brain/heart, solids from infusion...........................8.0g
Peptic digest of animal tissue .............................5.0g
NaCl........................................................5.0g
Glucose.....................................................2.0g
Na2HPO4 ....................................................2.5g
Sheep blood, defibrinated.................................50.0mL
Kanamycin solution........................................10.0mL
pH 7.4 ± 0.2 at 25°C
Kanamycin Solution:
Composition per 10.0mL:
Kanamycin................................................25.0mg
Preparation of Kanamycin Solution: Add kanamycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except kanamycin so-
lution and sheep blood, to distilled/deionized water and bring volume
to 940.0mL. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti-
cally add sterile kanamycin solution and sheep blood. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of fastidious fungi.