Brain Heart Infusion Agar with Penicillin and Streptomycin

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar
with Penicillin and Streptomycin

培养基配方
Composition per liter:
Pancreatic digest of casein..................................16.0g
Agar ........................................................13.5g
Brain heart, solids from infusion ...........................8.0g
Peptic digest of animal tissue...............................5.0g
NaCl.........................................................5.0g
Glucose .....................................................2.0g
Na2HPO4 .....................................................2.5g
Streptomycin...............................................40.0mg
Penicillin................................................20,000U
Sheep blood, defibrinated..................................50.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly and while stirring bring to a boil for 1 min to completely dissolve.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally add 50.0mL of defibrinated sheep blood. Mix thoroughly. Pour
into sterile Petri dishes while agitating gently to distribute the precipi-
tate through the medium.
Use: For the isolation and cultivation of a wide variety of fungal species,
especially systemic fungi, from clinical and nonclinical specimens. For
the selective isolation of pathogenic fungi from specimens heavily con-
taminated with bacteria and saprophytic fungi. For the maintenance of
fungal species on slant cultures.