Brain Heart Infusion Agar with 10% Sheep Blood, Gentamicin, and Chloramphenicol

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with
10% Sheep Blood, Gentamicin,
and Chloramphenicol

培养基配方
Composition per liter:
Pancreatic digest of casein................................16.0g
Agar ......................................................13.5g
Brain heart, solids from infusion ..........................8.0g
Peptic digest of animal tissue..............................5.0g
NaCl........................................................5.0g
Glucose ....................................................2.0g
Na2HPO4 ....................................................2.5g
Sheep blood, defibrinated................................100.0mL
Antibiotic solution ......................................10.0mL
pH 7.4 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Chloramphenicol...........................................0.05g
Gentamicin................................................0.05g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except antibiotic solu-
tion and sheep blood, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile antibiotic solution and sheep blood. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of a wide variety of fungal species,
especially systemic fungi, from clinical and nonclinical specimens. For
the selective isolation of pathogenic fungi from specimens heavily con-
taminated with bacteria and saprophytic fungi. For the maintenance of
fungal species on slant cultures.