Brain Heart Infusion Agar with Tween™ 80 (ATCC Medium 1941)

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with Tween™ 80
(ATCC Medium 1941)

培养基配方
Composition per liter:
Pancreatic digest of casein....................................16.0g
Agar...........................................................13.5g
Brain heart, solids from infusion...............................8.0g
Peptic digest of animal tissue .................................5.0g
NaCl............................................................5.0g
Glucose.........................................................2.0g
Na2HPO4 ........................................................2.5g
Tween™ 80.......................................................1.0g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks while shaking to distribute precipitate. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation of Helicococcus kunzii.