Brain Heart Infusion Agar with Vitamin B 12 (LMG Medium 207)

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with Vitamin B 12
(LMG Medium 207)

培养基配方
Composition per 1100.1mL:
Brain heart infusion agar ..................................1.0L
Vitamin B 12 solution....................................100.0mL
pH 7.4 ± 0.2 at 25°C
Brain Heart Infusion Agar:
Composition per liter:
Beef heart infusion.....................................250.0g
Calf brain infusion.....................................200.0g
Agar.....................................................13.5g
Proteose peptone.........................................10.0g
NaCl......................................................5.0g
Na2HPO4 ·12H2O...........................................2.5g
Glucose...................................................2.0g
Preparation of Brain Heart Infusion Agar: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Vitamin B 12 Solution:
Composition per 100.0mL:
Vitamin B 12 ...........................................0.1mg
Preparation of Vitamin B 12 Solution: Add Vitamin B 12 to
100.0mL of distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically add 100.01mL vitamin B 12
solution to 1.0L sterile brain heart infusion agar. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Bacillus sporothermodurans.