Brain Heart Infusion with Agar, Yeast Extract, Sodi- um Chloride, Inactivated Horse Serum, and Penicillin

[所属分类:培养基配方] [发布时间:2020-1-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Agar, Yeast Extract, Sodi-
um Chloride, Inactivated Horse Serum, and Penicillin

培养基配方
Composition per liter:
NaCl.....................................................20.0g
Pancreatic digest of gelatin.............................14.5g
Agar ....................................................12.0g
Brain heart, solids from infusion .......................6.0g
Peptic digest of animal tissue...........................6.0g
Yeast extract............................................5.0g
NaCl.....................................................5.0g
Glucose .................................................3.0g
Na2HPO4 .................................................2.5g
Horse serum, inactivated..............................100.0mL
Penicillin solution...................................10.0mL
pH 7.4 ± 0.2 at 25°C
Penicillin Solution:
Composition per 10.0mL:
Penicillin........................................1,000,000U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except penicillin solution
and inactivated horse serum, to distilled/deionized water and bring volume
to 890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add
sterile penicillin solution and horse serum. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of fastidious fungi.