Brain Heart Infusion Agar with Yeast Extract

[所属分类:培养基配方] [发布时间:2020-1-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar with Yeast Extract

培养基配方
Composition per liter:
Yeast extract..............................................20.0g
Pancreatic digest of casein................................16.0g
Agar ......................................................13.5g
Brain heart, solids from infusion ..........................8.0g
Peptic digest of animal tissue..............................5.0g
NaCl........................................................5.0g
Glucose ....................................................2.0g
Na 2 HPO 4 .................................................2.5g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath and BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks while shaking to distribute precipitate. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use:  For the cultivation of Mycoplasma equirhinis.