Brain Heart Infusion Blood Agar

[所属分类:培养基配方] [发布时间:2020-1-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Blood Agar

培养基配方
Composition per liter:
Blood agar....................................................1.1L
Overlay solution..............................................1.0L
pH 7.4 ± 0.2 at 25°C
Blood Agar:
Composition per liter:
Agar .........................................................18.0g
Pancreatic digest of gelatin..................................14.5g
Brain heart, solids from infusion ............................6.0g
Peptic digest of animal tissue................................6.0g
NaCl..........................................................5.0g
Glucose ......................................................3.0g
Na2HPO4 ......................................................2.5g
Rabbit blood, defibrinated ................................100.0mL
Preparation of Blood Agar:  Add components, except rabbit
blood, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 7.4. Distrib-
ute 5.0mL of agar solution into tubes. Autoclave for 25 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 0.5mL of sterile rabbit
blood to each tube. Mix thoroughly. Allow tubes to cool in a slanted po-
sition.
Overlay Solution:
Composition per liter:
Pancreatic digest of gelatin...................................14.5g
Brain heart, solids from infusion .............................6.0g
Peptic digest of animal tissue.................................6.0g
NaCl...........................................................5.0g
Glucose .......................................................3.0g
Na2HPO4 .......................................................2.5g
Preparation of Overlay Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Prior to inoculation, add 0.5mL of over-
lay solution to the surface of solidified blood agar slants.
Use: For the cultivation of Blastocrithidia leptocoridis, Leishmania
aethiopica, Leishmania amazonensis, Leishmania aristedesi, Leishma-
nia braziliensis, Leishmania chagasi, Leishmania donovani, Leishma-
nia enriettii, Leishmania garnhami, Leishmania gerbilli, Leishmania
guyanensis, Leishmania hertigi, Leishmania infantum, Leishmania
major, Leishmania mexicana, Leishmania panamensis, Leishmania
pifanoi, Leishmania tropica, and Trypanosoma conorrhini.