Brain Heart Infusion with Chicken Serum

[所属分类:培养基配方] [发布时间:2020-1-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Chicken Serum

培养基配方
Composition per liter:
Yeast extract..............................................20.0g
Pancreatic digest of casein................................16.0g
Brain heart, solids from infusion..........................8.0g
Peptic digest of animal tissue ............................5.0g
NaCl.......................................................5.0g
Na2HPO4 ...................................................2.5g
Glucose....................................................2.0g
Chicken serum, heat inactivated..........................50.0mL
Nicotinamide adenine dinucleotide solution ..............10.0mL
pH 7.2 ± 0.2 at 25°C
Nicotinamide Adenine Dinucleotide Solution:
Composition per 10.0mL:
Nicotinamide adenine dinucleotide ........................0.1g
Preparation of Nicotinamide Adenine Dinucleotide Solution:
Add 0.1g of nicotinamide adenine dinucleotide to distilled/deinonized
water and bring volume to 10.0mL Filter sterilize.
Preparation of Medium: Add components, except chicken serum
and nicotinamide adenine dinucleotide solution, to distilled/deionized
water and bring volume to 940.0mL. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 10.0mL. of nico-
tinamide adenine dinucleotide solution and 50.0mL of heat-inactivated
chicken serum. Mix thoroughly. Aseptically distribute into sterile tubes
or flasks.
Use:  For the cultivation of Haemophilus paragallinarum and Pasteur-
ella avium.