Brain Heart Infusion with Erythromycin (BHI with Erythromycin) (DSMZ Medium 215a)

[所属分类:培养基配方] [发布时间:2020-1-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Erythromycin
(BHI with Erythromycin)
(DSMZ Medium 215a)

培养基配方
Composition per liter:
Pancreatic digest of gelatin..................................14.5g
Brain heart, solids from infusion .............................6.0g
Peptic digest of animal tissue.................................6.0g
NaCl...........................................................5.0g
Glucose .......................................................3.0g
Na2HPO4 .......................................................2.5g
Erythromycin solution .......................................10.0mL
pH 7.4 ± 0.2 at 25°C
Erythromycin Solution:
Composition per 10.0mL:
Erythromycin...............................................10.0mg
Preparation of Erythromycin Solution: Add erythromycin to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except erythromycin
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
erythromycin solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation and cultivation of Nocardia crassostreae.