Brain Heart Infusion with Glucose and Horse Serum (LMG Medium 183)

[所属分类:培养基配方] [发布时间:2020-1-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Glucose and Horse Serum
(LMG Medium 183)

培养基,培养基配方
Composition per 1300.0mL:
Brain heart infusion agar ...................................1.0L
Horse serum...............................................200.0mL
Glucose solution..........................................100.0mL
pH 7.4 ± 0.2 at 25°C
Brain Heart Infusion Agar:
Composition per liter:
Beef heart infusion........................................250.0g
Calf brain infusion........................................200.0g
Agar........................................................13.5g
Proteose peptone............................................10.0g
NaCl.........................................................5.0g
Na2HPO4 ·12H2O..............................................2.5g
Glucose......................................................2.0g
Preparation of Brain Heart Infusion Agar: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Glucose Solution:
Composition per 100.0mL:
Glucose......................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Aseptically add 200.0mL sterile horse se-
rum and 100.0mL sterile glucose solution to 1.0L sterile brain heart in-
fusion agar. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation of Streptococcus pyogenes.