Brain Heart Infusion with Horse Blood (LMG Medium 231)

[所属分类:培养基配方] [发布时间:2020-1-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Horse Blood
(LMG Medium 231)

培养基配方
Composition per liter:
Beef heart infusion.........................................250.0g
Calf brain infusion.........................................200.0g
Agar.........................................................13.5g
Proteose peptone.............................................10.0g
NaCl..........................................................5.0g
Na2HPO4 ·12H2O...............................................2.5g
Glucose.......................................................2.0g
Horse blood.................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 950.0L. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile horse
blood. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the cultivation of Helcococcus kunzii and Globicatella sul-
fidifaciens.