Brain Heart Infusion Soil Extract Medium

[所属分类:培养基配方] [发布时间:2020-1-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Soil Extract Medium

培养基配方
Composition per liter:
Yeast extract...................................................20.0g
Pancreatic digest of casein.....................................16.0g
Brain heart, solids from infusion ...............................8.0g
Peptic digest of animal tissue...................................5.0g
NaCl.............................................................5.0g
Na2HPO4 .........................................................2.5g
Glucose .........................................................2.0g
Soil extract..................................................250.0mL
Vitamin B 12 solution...........................................1.0mL
pH 7.2 ± 0.2 at 25°C
Soil Extract:
Composition per 400.0mL:
African Violet soil..............................................1.0g
Na2CO3 ..........................................................1.0g
Preparation of Soil Extract: Autoclave for 60 min at 15 psi pres-
sure–121°C. Filter through paper before using in medium.
Vitamin B 12 Solution:
Composition per 1.0mL:
Vitamin B 12 ..................................................2.0μg
Preparation of Vitamin B 12 Solution: Add vitamin B 12 to dis-
tilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Fil-
ter sterilize.
Preparation of Medium: Add components, except glucose, yeast
extract, and vitamin B 12 solution, to tap water and bring volume to
799.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Add yeast extract and glucose to 200.0mL of tap water. Filter
sterilize and add aseptically to cooled, sterile basal medium. Aseptical-
ly add 1.0mL of vitamin B 12 solution. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks.
Use:  For the cultivation of a wide variety of microorganisms, includ-
ing bacteria, yeasts, and molds, especially fastidious species from soil.
For the isolation of Histoplasma capsulatum and other pathogenic
fungi, including Coccidioides immitis.