Brain Liver Heart Semisolid Medium

[所属分类:培养基配方] [发布时间:2020-1-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Liver Heart Semisolid Medium

培养基配方
Composition per liter:
Beef heart, infusion from......................................250.0g
Calf brains, infusion from.....................................200.0g
Liver, infusion from...........................................50.0g
Proteose peptone...............................................10.0g
NaCl............................................................5.0g
Neopeptone.....................................................3.25g
Pancreatic digest of casein....................................3.25g
Na2HPO4 ........................................................2.5g
Glucose.........................................................2.0g
Agar...........................................................1.75g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Leave in tubes.
Use:  For the cultivation of fastidious microorganisms. For the cultiva-
tion of Actinomyces bovis, Actinomyces israelii, Actinomyces naeslundii,
Clostridium symbiosum, and Eubacterium limosum.
Brettanomyces Agar Base with Selective Supplement
Composition per liter:
Agar.............................................................20.0g
Glucose..........................................................10.0g
Peptic digest of animal tissue ...................................5.0g
Yeast extract.....................................................3.0g
Malt extract......................................................3.0g
Chloramphenical...................................................0.1g
Cycloheximide.....................................................0.1g
Thiamine hydrochloride...........................................0.01g
Selective supplement solution ..................................10.0mL
Source: This medium is available from HiMedia.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Selective Supplement Solution:
Composition per 10.0mL:
Chlortetracycline.................................................0.1g
Gentamicin.......................................................0.05g
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution. .
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the cultivation of Brettanomyces spp. and the detection of
this yeast in soft drinks.