Brewer Thioglycollate Medium, Modified

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brewer Thioglycollate Medium, Modified

培养基配方
Composition per liter:
Casein enzymatic hydrolysate ..............................17.5g
Glucose....................................................10.0g
NaCl........................................................5.0g
Papaic digest of soybean meal...............................2.5g
K2HPO4 .....................................................2.0g
Na-thioglycollate ..........................................1.0g
Agar........................................................0.5g
Methylene Blue.............................................2.0mg
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat to boiling.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation and maintenance of anaerobic and microaero-
philic microorganisms. For testing the sterility of biological products
and materials.