Brigg’s Liver Broth pH 7.0

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brigg’s Liver Broth pH 7.0
Composition per liter:
Glucose ...................................................20.0g
Neopeptone.................................................15.0g
Yeast extract...............................................6.0g
NaCl........................................................5.0g
Tween™ 80...................................................1.0g
Soluble starch..............................................0.5g
L -Cysteine·HCl·H2O ......................................0.2g
Tomato juice solution....................................400.0mL
Liver extract.............................................75.0mL
pH 7.0 ± 0.2 at 25°C
Tomato Juice Solution:
Composition per 500.0mL:
Tomato juice.............................................250.0mL
Preparation of Tomato Juice Solution:  Add 250.0mL of tomato
juice to distilled/deionized water and bring volume to 500.0mL. Mix
thoroughly. Adjust pH to 7.0 with 10% NaOH. Filter through Whatman
#2 filter paper.
Liver Extract:
Composition per 170.0mL:
Liver powder....................................................10.0g
Preparation of Liver Extract:  Add 10.0g of liver powder to
170.0mL of distilled/deionized water. Gently heat to 60°C. Maintain at
50°–60°C for 1 hr. Gently bring to boiling. Boil for 5 min. Adjust pH
to 7.2. Filter through Whatman #2 filter paper.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Lactobacillus acetotolerans.